Tag
KILR

Part:BBa_M10037:Design

Designed by: Patrick Harrigan   Group: Berkeley BioE140L - S09   (2009-05-10)

{˂KILR˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

The part is a synthetic construct encoding the protein sequence: VKELEDKNEELLSkiYHlrNEVARLKKLVGERGGC

Construction of {<KILR>} basic part

Wobble Oph024F/Oph025R        (136 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5   (EcoRI/BamHI, L)
Product is pBca9495AK-M10037    {<KILR>}<br>
----
Oph024F        foward oligo for cloning of {<KILR>}
ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025R        reverse oligo for cloning of {<KILR>}
ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc

References

Havranek J, Harbury, P. Automated design of specificity in molecular recognition. Nature Structural Biology 10, 45 - 52 (2002)