Tag
KILR
Part:BBa_M10037:Design
Designed by: Patrick Harrigan Group: Berkeley BioE140L - S09 (2009-05-10)
{˂KILR˃}
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
The part is a synthetic construct encoding the protein sequence: VKELEDKNEELLSkiYHlrNEVARLKKLVGERGGC
Construction of {<KILR>} basic part Wobble Oph024F/Oph025R (136 bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L) Product is pBca9495AK-M10037 {<KILR>}<br> ---- Oph024F foward oligo for cloning of {<KILR>} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg Oph025R reverse oligo for cloning of {<KILR>} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc
References
Havranek J, Harbury, P. Automated design of specificity in molecular recognition. Nature Structural Biology 10, 45 - 52 (2002)